Abstract: The aquaculture industry has, during the last decade, replaced the feed ingredients from fish meal and fish oil with plant based alternatives. Negative effects on growth performance and flesh quality are observed, but how the fish feed compositi on affect the epigenetic regulation of gene transcription during the different life stages and across generations remains an important and unexplored field. Epigenetics encompasses changes in gene expression profiles without alterations in the genomic DNA sequence. These changes arise from the inherited epigenetic regulation of the gene, but are also sensitive to environmental factors like nutrition. This project will answer how feed enriched with methyl donors regulates gene expression patterns by DNA me thylation, directly and across generations in fish. Zebrafish will be used as a model prior to studies on Atlantic salmon. To date there are no studies on nutritional epigenetic regulation in fish, and through this project we establish advanced epigenetic procedures for transgenerationl fish studies.
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