The world production of fish oil, the major source of the essential fatty acids EPA and DHA, has been stable since the 1970s. Due to increasing demands for EPA and DHA as ingredients in human health products and fish feed, there is now a serious shortage of these fatty on the international markets. This has led to increasing substitution of fish oil by plant oils in Atlantic salmon feed and thereby reducing the EPA and DHA content of salmon. This reduction is probably unavoidable, so it is urgently neede d to know the minimum required levels of these fatty acids in fish diets for securing health and quality. It is known that 75-100% of the fish oil can be substituted by plant oils without negative effects on growth, as long as the diet contains fish meal . Today the level of fish meal in salmon diets is also decreasing leading to an even more pronounced reduction in EPA and DHA. This project will determine the minimum EPA and DHA requirements in diets for modern Atlantic salmon, with fast growth and low f eed conversion ratio, at different life stages in sea-water. The project will further assess if the capacity to produce EPA and DHA from 18:3n-3 is influenced by different dietary fatty acids, bioactive components and temperature. This project will use n ew advanced technologies to define some early symptoms of EPA and DHA deficiency, since sub-deficiency states can result in increased health risks, even when not manifested by overt symptoms and reduced growth.
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